Dr. Alexander Loy
Department of Microbial Ecology
Faculty Center of Ecology
Faculty of Life Sciences
University of Vienna
Althanstr. 14
A-1090 Wien
Austria
 
phone: +43 1 4277 57317
fax: +43 1 4277 54389
email:
 
Topics
 
 Ribosomal RNA-targeted oligonucleotide microarrays (PhyloChips) for analysis of microbial communities
 Diversity, ecophysiology, and evolution of sulfate-reducing microbes
 DNA Microarray technology for biodiversity inventories of sulfate reducing prokaryotes (Ph.D. thesis as PDF document)
 Ecology and genetics of the intestinal microbiota in health and disease (InflammoBiota)
 
Publications in peer reviewed journals
  1. Pester M, Rattei T, Flechl S, Gröngröft A, Richter A, Overmann J, Reinhold-Hurek B, Loy A, Wagner M. 2012. amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions. Environ. Microbiol. , in press.
  2. Berry D, Ben Mahfoudh K, Wagner M, Loy A. 2011. Barcoded primers used in multiplex amplicon pyrosequencing bias amplification. Appl. Environ. Microbiol. 77: 7846-7849.
     PUBMED
     Supplementary material [PDF]
  3. Steger D, Berry D, Haider S, Horn M, Wagner M, Stocker R, Loy A. 2011. Systematic spatial bias in DNA microarray hybridization is caused by probe spot position-dependent variability in lateral diffusion. PloS One 6: e23727.
     Full text    PUBMED
  4. Gruber-Vodicka HR, Dirks U, Leisch N, Baranyi C, Stoecker K, Bulgheresi S, Heindl NR, Horn M, Lott C, Loy A, Wagner M, Ott J. 2011. Paracatenula, an ancient symbiosis between thiotrophic Alphaproteobacteria and catenulid flatworms. Proc. Natl. Acad. Sci. USA 108: 12078-12083.
     PUBMED
  5. Steger D, Wentrup C, Braunegger C, Deevong P, Hofer M, Richter A, Baranyi C, Pester M, Wagner M, Loy A. 2011. Microorganisms with novel dissimilatory (bi)sulfite reductase genes are widespread and part of the core microbiota in low-sulfate peatlands. Appl .Environ. Microbiol. 77: 1231-1242.
     PUBMED
     Supplementary material [PDF]
  6. Pester M, Bittner N, Deevong P, Wagner M, Loy A. 2010. A 'rare biosphere' microorganism contributes to sulfate reduction in a peatland. ISME J. 12: 1591-1602.
     PUBMED
     Supplementary information [PDF]
  7. Hubert C, Arnosti C, Brüchert V, Loy A, Vandieken V, Jørgensen BB. 2010. Thermophilic anaerobes in Arctic marine sediments induced to mineralize complex organic matter at high temperature. Environ. Microbiol. 12: 1089-1104.
     PUBMED
  8. Hubert C, Loy A, Nickel M, Arnosti C, Baranyi C, Brüchert V, Ferdelman T, Finster K, Christensen FM, Rosa de Rezende J, Vandieken V, Jørgensen BB. 2009. A constant flux of diverse thermophilic bacteria into the cold Arctic seabed. Science 325: 1541-1544.
     PUBMED
  9. Hesselsøe M, Füreder S, Schloter M, Bodrossy L, Iversen N, Roslev P, Nielsen PH, Wagner M, Loy A. 2009. Isotope array analysis of Rhodocyclales uncovers functional redundancy and versatility in an activated sludge. ISME J. 3: 1349-1364.
     PUBMED
  10. Rinke C, Schmitz-Esser S, Loy A, Horn M, Wagner M, Bright M. 2009. High genetic similarity between two geographically distinct strains of the sulfur-oxidizing symbiont 'Candidatus Thiobios zoothamnicoli' FEMS Microbiol. Ecol. 67: 229-41.
     PUBMED
  11. Loy A, Duller S, Baranyi C, Mußmann M, Ott J, Sharon I, Béjà O, Le Paslier D, Dahl C, Wagner M. 2009. Reverse dissimilatory sulfite reductase as phylogenetic marker for a subgroup of sulfur-oxidizing prokaryotes. Environ. Microbiol. 11: 289-299.
     PUBMED
     Supplementary figure 1
     Supplementary table 1
     DsrAB database for ARB
  12. Schönmann S, Loy A, Wimmersberger C, Sobek J, Rehrauer H, Frey B, Vandamme P, Eberl L. 2009. 16S rRNA gene-based phylogenetic microarray for simultaneous identification of members of the genus Burkholderia. Environ. Microbiol. 11: 779-800.
     PUBMED
  13. Loy A, Arnold R, Tischler P, Rattei T, Wagner M, Horn M. 2008. probeCheck - a central resource for evaluating oligonucleotide probe coverage and specificity. Environ. Microbiol. 10: 2894-2896.
     PUBMED
  14. Ruehland C, Blazejak A, Lott C, Loy A, Erseus C, Dubilier N. 2008. Multiple bacterial symbionts in two species of co-occurring gutless oligochaete worms from Mediterranean sea grass sediments. Environ Microbiol. 10: 3404-3416.
     PUBMED
  15. Miletto M, Loy A, Antheunisse AM, Loeb R, Bodelier PLE, Laanbroek HJ. 2008. Biogeography of sulfate-reducing prokaryotes in river floodplains. FEMS Microbiol. Ecol. 64: 395-406.
     PUBMED
  16. Wagner M, Smidt H, Loy A, Zhou J. 2007. Unravelling microbial communities with DNA-microarrays: challenges and future directions. Microb. Ecol. 53: 498-506.
     PUBMED
  17. Lücker S, Steger D, Kjeldsen KU, MacGregor BJ, Wagner M, Loy A. 2007. Improved 16S rRNA-targeted oligonucleotide probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization. J. Microbiol. Methods. 69: 523-528.
     PUBMED
     Supplementary online material
  18. Kjeldsen KU, Loy A, Thomsen TR, Jakobsen TF, Wagner M, and Ingvorsen K. 2007. Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment in Great Salt Lake (Utah, USA). FEMS Microbiol. Ecol. 60: 287-298.
     PUBMED
  19. Loy A, Maixner F, Wagner M, Horn M. 2007. probeBase - an online resource for rRNA-targeted oligonucleotide probes: new features 2007. Nucleic Acids Res. 35: D800-D804.
     PUBMED
  20. Leloup J, Loy A, Knab NJ, Borowski C, Wagner M, Jørgensen BB. 2007. Diversity and abundance of sulfate-reducing microorganisms in the sulfate and methane zones of a marine sediment, Black Sea. Environ. Microbiol. 9: 131-142.
     PUBMED
  21. Imachi H, Sekiguchi Y, Kamagata Y, Loy A, Qiu YL, Hugenholtz P, Kimura N, Wagner M, Ohashi A, Harada H. 2006. Non-sulfate-reducing, syntrophic bacteria affiliated with the Desulfotomaculum cluster I are widely distributed in methanogenic environments. Appl. Environ. Microbiol. 72: 2080-2091.
     PUBMED
  22. Wagner M, Nielsen PH, Loy A, Nielsen JL, Daims H. 2006. Linking microbial community structure with function: fluorescence in situ hybridization-microautoradiography and isotope arrays. Curr. Opin. Biotechnol. 17: 1-9.
     PUBMED
  23. Loy A, Bodrossy L. 2006. Highly parallel microbial diagnostics using oligonucleotide microarrays. Clinica Chimica Acta 363: 106-119.
     PUBMED
  24. Sabehi G, Loy A, Jung KH, Partha R, Spudich JL, Isaacson T, Hirschberg J, Wagner M, Beja O. 2005. New insights into metabolic properties of marine bacteria encoding proteorhodopsins. PLoS Biol. 3: e273.
     PUBMED
  25. Lehner A, Loy A, Behr T, Gaenge H, Ludwig W, Wagner M, Schleifer KH. 2005. Oligonucleotide microarray for identification of Enterococcus species. FEMS Microbiol. Lett. 246: 133-142.
     PUBMED
  26. Wagner M, Loy A, Klein M, Lee N,Ramsing NB, Stahl DA, Friedrich MW. 2005. Functional marker genes for identification of sulphate-reducing prokaryotes. Methods Enzymol. 397: 469-489.
     PUBMED
  27. Loy A, Beisker W, Meier H. 2005. Diversity of bacteria growing in a natural mineral water after bottling. Appl. Environ. Microbiol. 71: 3624-3632.
     PUBMED
  28. Zverlov V, Klein M, Lücker S, Friedrich MW, Kellermann J, Stahl DA, Loy A, Wagner M. 2005. Lateral gene transfer of dissimilatory (bi)sulfite reductase revisited. J. Bacteriol. 187: 2203-2208.
     PUBMED
  29. Loy A, Schulz C, Lücker S, Schöpfer-Wendels A, Stoecker K, Baranyi C, Lehner A, Wagner M. 2005. 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order Rhodocyclales. Appl. Environ. Microbiol. 71: 1373-1386.
     PUBMED
  30. Loy A, Küsel K, Lehner A, Drake HL, Wagner M. 2004. Microarray and functional gene analyses of sulfate-reducing prokaryotes in low sulfate, acidic fens reveal co-occurrence of recognized genera and novel lineages. Appl. Environ. Microbiol. 70: 6998-7009.
     PUBMED
  31. Loy A, Horn M, Wagner M.. 2003. probeBase - an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res. 31: 514-516.
     PUBMED
  32. Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M. 2002. Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Appl. Environ. Microbiol. 68: 5064-5081.
     PUBMED
  33. Wagner M, Loy A, Nogueira R, Purkhold U, Lee N, Daims H. 2002. Microbial community composition and function in wastewater treatment plants. Antonie van Leeuwenhoek 81: 665-680 .
     PUBMED
  34. Wagner M, Loy A. 2002. Bacterial community composition and function in sewage treatment systems. Curr. Opin. Biotechnol. 13: 218-227.
     PUBMED
  35. Juretschko S, Loy A, Lehner A, Wagner M. 2002. The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach. Syst. Appl. Microbiol. 25: 84-99.
     PUBMED

Book chapters and other publications
  1. Loy A, Pester M, Steger D. 2011. Phylogenetic microarrays for cultivation-independent identification and metabolic characterization of microorganisms in complex samples. In PCR mutation detection protocols. Methods in Molecular Biolology (Vol. 688), pp. 187-206. (Theophilus BDM and Rapley R, ed.). Springer, Dordrecht Heidelberg London, New York.
  2. Loy A, Pester M. 2010. Probing identity and physiology of uncultured microorganisms with isotope labeling techniques. In Geomicrobiology: Molecular and Environmental Perspective, pp. 127-146. (Barton LL, Mandl M, Loy A, ed.). Springer, Dordrecht, Heidelberg, London, New York.
  3. Taylor MW, Loy A, and Wagner M. 2010. Microarrays for studying the composition and function of microbial communities. In The microbiology of activated sludge, pp. 397-411. (Seviour RJ, Nielsen PH, ed.). IWA Publishing, London, UK.
  4. Loy A, Duller S, and Wagner M.. 2008. Evolution and ecology of microbes dissimilating sulfur compounds: Insights from siroheme sulfite reductases. In Microbial Sulfur Metabolism, pp. 46-59. (Friedrich C, Dahl C, ed.). Springer, New York.
  5. Stahl DA, Loy A, Wagner M. 2007. Molecular strategies for studies of natural populations of sulphate-reducing microorganisms. In Sulfate-reducing bacteria: Environmental and engineered systems, pp. 39-117. (Barton LL, Hamilton WA, ed.). Cambridge University Press, Cambridge, UK.
  6. Loy A, Taylor MW, Bodrossy L, Wagner M. 2006. Applications of nucleic acid microarrays in soil microbial ecology. In Molecular approaches to soil, rhizosphere and plant microorganism analysis, pp. 18-41. (Cooper JE, Rao JR, ed.). CABI Publishing, Wallingford, Oxfordshire, UK.
  7. Bodrossy L, Loy A. 2005. Oligonucleotide microarrays in microbial diagnostics. In Encyclopedia of medical genomics and proteomics, pp. 1-8. (Fuchs J, Podda M, ed.). Marcel Dekker Inc., New York, USA.
  8. Loy A, Wagner M. 2004. Analyse komplexer mikrobieller Lebensgemeinschaften mit phylogenetischen Mikroarrays. BioSpektrum 6: 745-747.
  9. Loy A, Daims H, Wagner M. 2002. Activated Sludge: Molecular techniques for determining community composition. In The Encyclopedia of Environmental Microbiology, pp. 26-43. (Bitton G, ed.). Wiley, New York .

 
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